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N-(3-二甲基氨基丙基)-N′-乙基碳二亞胺 鹽酸鹽

更新時(shí)間:2019-04-24 點(diǎn)擊次數(shù):857次

N-(3-二甲基氨基丙基)-N′-乙基碳二亞胺 鹽酸鹽

性質(zhì)

InChI Key  FPQQSJJWHUJYPU-UHFFFAOYSA-N
product line  BioXtra
impurities  ≤0.005% Phosphorus (P)
 ≤0.1% Insoluble matter
ign. residue  ≤0.1%
mp  110-115 °C (lit.)
solubility  H2O: 0.5 M, clear, colorless
anion traces  sulfate (SO42-): ≤0.05%
cation traces  Al: ≤0.0005%
 Ca: ≤0.0005%
 Cu: ≤0.0005%
 Fe: ≤0.0005%
 K: ≤0.005%
 Mg: ≤0.0005%
 NH4+: ≤0.05%
 Na: ≤0.005%
 Pb: ≤0.001%
 Zn: ≤0.0005%
storage temp.  −20°C

產(chǎn)品描述

包裝

1, 5, 10, 25 g in poly bottle

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
• for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
• for cross-linking of nanofiber matrices with mouse lung extract
• for activation of substrate XNA oligonucleotides
 

Biochem/physiol Actions

水溶性縮合試劑。EDAC通常用作與伯胺進(jìn)行酰胺鍵合的羧基活化劑。另外,它能與磷酸基團(tuán)反應(yīng)。其已用于例如肽合成、將蛋白質(zhì)與核酸交聯(lián)、以及免疫綴合物的制備。通常,EDAC在不含緩沖劑的pH范圍4.0-6.0的條件下使用。特別是,應(yīng)避免使用胺和羧酸鹽緩沖液。

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